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Vinculin, clone: 7F9, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9777-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15567676

  • £64.20 / 25µg


EU Stock 1
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Description

Description

Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.Applications Reported: This 7F9 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections and formalin-fixed paraffin embedded tissue sections, immunocytochemistry, and microscopy.Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on methanol-fixed or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 5 μg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval buffer and can be used at less than or equal to 5 μg/mL. The antibody has been tested by western blot on reduced and non-reduced cell lysates and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

vinculin
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P18206, Q64727, P85972
RP11-178G16.3, CMD1W, MVCL
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Western Blot
7F9
Unconjugated
VCL
Purified
VCL
IgG1, kappa
25μg
Store at 2-8°C.
Primary
7414
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