Vinculin, clone: 7F9, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9777-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.Applications Reported: This 7F9 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections and formalin-fixed paraffin embedded tissue sections, immunocytochemistry, and microscopy.Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on methanol-fixed or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 5 μg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval buffer and can be used at less than or equal to 5 μg/mL. The antibody has been tested by western blot on reduced and non-reduced cell lysates and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|P18206, Q64727, P85972|
|RP11-178G16.3, CMD1W, MVCL|
|ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þMicroscopyþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.