Vinculin Monoclonal Antibody (VLN01), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA511690

Product Code. 11394493

Description

MA5-11690 targets Vinculin in IF, IHC (P), IP, and WB applications and shows reactivity with Human samples. The MA5-11690 immunogen is semi-purified vinculin.

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

Specifications

• Antigen: Vinculin
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P18206, Q64727
• Gene Symbols: VCL
• Immunogen: Semi-purified vinculin
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Non-human Primate
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: VLN01
• Conjugate: Unconjugated
• Gene: VCL
• Gene Alias: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 22330, 7414
• Content And Storage: 4° C
• Form: Liquid