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Vimentin Antibody (VM452), Novus Biologicals™

Product Code. 18421342 Shop All Bio Techne Products
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0.1 mg
20 ug
Unit Size:
0.10mg
20µg
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Product Code. Quantity unitSize
18421342 20 ug 20µg
18154485 0.1 mg 0.10mg
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Product Code. 18421342 Supplier Novus Biologicals Supplier No. NBP22943320ug

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This item is not returnable. View return policy

Mouse Monoclonal Antibody has been used in 1 publication

Vimentin Monoclonal specifically detects Vimentin in Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Knockout Validated.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Vimentin
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone VM452
Concentration 0.2 mg/ml
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, Knockout Validated 0.7 ug/ml
Gene Accession No. P08670
Gene Alias FLJ36605, vimentin
Gene Symbols VIM
Host Species Mouse
Immunogen Recombinant full-length human vimentin protein (Uniprot: P08670)
Purification Method Protein A or G purified
Quantity 20 ug
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells
Primary or Secondary Primary
Gene ID (Entrez) 7431
Test Specificity This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.
Target Species Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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