Invitrogen™ Phospho-IRF3 (Ser396) Recombinant Rabbit Monoclonal Antibody (PSH06-75)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ MA557852

Product Code. 30284467

Description

Positive Control: HT-29 transfected with 2.5μg/mL Poly (I:C) for 6 hours cell lysate, J774A.1 transfected with 2.5μg/mL Poly (I:C) for 6 hours cell lysate, human testis tissue, HT-29 cells transfected withPoly (I:C). Tissue Specificity: Low tissue specificity. Subcellular Location: Cytoplasm, Nucleus, Mitochondrion. Sequence Similarities: 83% Mouse/Rat. Predicted band size: 47 kDa.

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

Specifications

• Antigen: Phospho-IRF3 (Ser396)
• Classification: Recombinant Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P70671, Q14653
• Gene Symbols: IRF3
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser396 of human IRF3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: PSH06-75
• Conjugate: Unconjugated
• Gene: IRF3
• Gene Alias: C920001K05Rik; IIAE7; Interferon regulatory factor 3; IRF3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3661, 54131
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid