Human/Mouse Active Caspase-3 Antibody, R&D Systems™

Rabbit Polyclonal Antibody has been used in 155 publications

Brand:  R&D Systems AF835

Product Code. 18011652

Description

Caspase-3 Polyclonal specifically detects Caspase-3 in Human, Mouse samples. It is validated for Immunohistochemistry, Immunocytochemistry.

Specifications

• Antigen: Caspase-3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Accession No.: P42574
• Gene Symbols: CASP3
• Immunogen: KLH-coupled human Caspase-3 synthetic peptide CRGTELDCGIETD Accession # P42574
• Quantity: 50 μg
• Primary or Secondary: Primary
• Test Specificity: Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
• Target Species: Human, Mouse
• Isotype: IgG

• Applications: Immunohistochemistry, Immunocytochemistry
• Concentration: LYOPH
• Dilution: Immunohistochemistry 1-15 ug/mL, Immunocytochemistry 5-15 ug/mL
• Gene Alias: Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 836
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.