PeproTech VEGF-165 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17893953	PeproTech 500-P10BT-25UG	25 μg
17853913	PeproTech 500-P10BT-50UG	50 μg
17804423	Invitrogen 500-P10BT-1MG	2 x 500μg
30054679	PeproTech 500-P10BT-500UG	500 μg

Description

AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF 165 (500-P10) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P10BT-1MG will be provided as 2 x 500 μg

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

Specifications

• Antigen: VEGF-165
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P15692
• Gene Symbols: Vegfa
• Primary or Secondary: Primary
• Content And Storage: -20°C

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene ID (Entrez): 7422
• Immunogen: E.coli-derived, 38.2 kDa Recombinant Human VEGF 165.
• Purification Method: Antigen affinity chromatography
• Gene: Vegfa