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Invitrogen™ V5 Tag Monoclonal Antibody, FITC

Product Code. 10719204
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10719204 50 μL Each
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For Research Use Only. All usage must comply with product instructions.
 
Product Code. 10719204 Supplier Invitrogen™ Supplier No. R96325

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294.12 GBP valid until 2026-04-10
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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Mouse Monoclonal Antibody

NOTE: R963-25 was previously sold under product #46-0308. When re-ordering, please use #R963-25. R963-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. This antibody has been tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins. For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS). The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. This antibody has also been tested against purified Positope™ control protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
TRUSTED_SUSTAINABILITY

Specifications

Antigen V5 Tag
Applications Western Blot, Immunocytochemistry
Classification Monoclonal
Concentration 1 mg/mL
Conjugate FITC
Formulation PBS with 0.01% sodium azide
Gene Alias simian virus 5 antibody; SPV5gp2; sv5; V5; v-5; V5 Epitope Tag
Host Species Mouse
Immunogen V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.
Purification Method Purified
Quantity 50 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgG2a
Target Molecule Tag
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