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TCR V beta 8.1/8.2, PerCP-eFluor™ 710, clone: KJ16-133 (KJ-16, KJI6, KJ-I6), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5813-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15381360

  • £70.58 / 25µg


EU Stock 1
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Description

Description

Description: This KJ16-133 monoclonal antibody recognizes mouse T cell receptors (TCR) containing Vbeta 8.1 or 8.2, which is expressed by 15-20% of peripheral T cells in most strains of mice (ie. Balb/c, AKR, C57BL/6, B10.A) and by a number of T cell hybridomas (ie. DO-11.10, 3DT-52.5, D1G10G11, SKK-9.11, SKK-45.10, MDK16, S18.4).Applications Reported: This KJ16-133 (KJ-16, KJI6, KJ-I6) antibody has been reported for use in flow cytometric analysis.Applications Tested: This KJ16-133 (KJ-16, KJI6, KJ-I6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

V beta 8.1, 8.2 TCR
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
TCRV beta 8.1, TCRV beta8.1, TCR V beta8.1, TCR V beta8.2, Vb8.1, Vbeta8.1, Vb8.2, Vbeta8.2
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Murine
Flow Cytometry
KJ16-133 (KJ-16, KJI6, KJ-I6)
PerCP-eFluor 710
Conjugated
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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