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TCR V beta 8.1/8.2 Monoclonal Antibody (KJ16-133 (KJ-16, KJI6, KJ-I6)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5813-80
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: This KJ16-133 monoclonal antibody recognizes mouse T cell receptors (TCR) containing Vbeta 8.1 or 8.2, which is expressed by 15-20% of peripheral T cells in most strains of mice (ie. Balb/c, AKR, C57BL/6, B10.A) and by a number of T cell hybridomas (ie. DO-11.10, 3DT-52.5, D1G10G11, SKK-9.11, SKK-45.10, MDK16, S18.4). Applications Reported: This KJ16-133 (KJ-16, KJI6, KJ-I6) antibody has been reported for use in flow cytometric analysis. Applications Tested: This KJ16-133 (KJ-16, KJI6, KJ-I6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
TCR V beta 8.1/8.2 Monoclonal antibody specifically detects TCR V beta 8.1/8.2 in Mouse samples. It is validated for Flow Cytometry.Specifications
TCR V beta 8.1/8.2 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Rat | |
25 μg | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
KJ16-133 (KJ-16, KJI6, KJ-I6), KJI6, KJ-I6) | |
PerCP-eFluor 710 | |
TCR V beta8.1; TCR V beta8.2; TCRV beta 8.1; TCRV beta8.1; Vb8.1; Vb8.2; Vbeta8.1; Vbeta8.2 | |
Affinity chromatography | |
RUO | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.