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TCR V alpha 2 Monoclonal Antibody (B20.1), Biotin, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen 13-5812-81
Description
Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology. Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 μg of purified anti-mouse CD16/32 (cat. 14-0161). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.Specifications
TCR V alpha 2 | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Rat | |
50 μg | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry, Immunoprecipitation | |
B20.1 | |
Biotin | |
TCRVA; TCRVA2; V alpha 2; V alpha-2 TCR; Va 2; Va2; Valpha 2; Valpha2 | |
Affinity chromatography | |
RUO | |
Mouse | |
Antibody | |
IgG2a λ |
For Research Use Only.