USP7 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA534911

Product Code. 15378971

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, USP7 transfected 293T lysate, NIH-3T3, JC, PC-12. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (93%), Pig (97%), Chicken (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

Specifications

• Antigen: USP7
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: USP7
• Gene Alias: 2210010O09Rik; AA409944; AA617399; AU019296; AW548146; C80752; deubiquitinating enzyme 7; Hausp; Herpes virus-associated ubiquitin-specific protease; herpes-virus-associated ubiquitin-specific protease; herpesvirus-associated ubiquitin-specific protease; mHAUSP; protease; rHAUSP; TEF1; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7; ubiquitin specific protease 7 (herpes virus-associated); ubiquitin thioesterase 7; ubiquitin thiolesterase 7; ubiquitin-specific-processing protease 7; UBP; USP7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 252870, 360471, 7874
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 0.94 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q4VSI4, Q6A4J8, Q93009
• Gene Symbols: USP7
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 221 of USP7 (Uniprot ID#Q93009)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG