Invitrogen™ USP3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA585512

Product Code. 16372445

Description

Keep as concentrated solution. Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (87%), Rhesus Monkey (99%). Positive Control: 293T, A431, HeLa, human USP3-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Specifications

• Antigen: USP3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: USP3
• Gene Alias: AA409661; BC017156; Deubiquitinating enzyme 3; SIH003; Ubiquitin carboxyl-terminal hydrolase 3; ubiquitin specific peptidase 3; ubiquitin specific protease 3; ubiquitin thioesterase 3; ubiquitin thiolesterase 3; ubiquitin-specific-processing protease 3; UBP; Usp3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9960
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9Y6I4
• Gene Symbols: USP3
• Immunogen: Recombinant protein encompassing a sequence within the center region of human USP3. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG