Invitrogen™ USP25 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA588642

Product Code. 16506821

Description

Immunogen sequence: MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA VAFLTAKNAK TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD DLQRAIALSL AESNRAFRET GITDEEQAIS RVLEASIAEN KACLKRTPTE VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF SAVIQSLFNL LEFRRLVLNY KPPSNAQDLP RNQKEHRNLP FMRELRYLFA; Positive Samples: HL-60, HT1080, U251MG, HeLa, SKOV3, Mouse testis, Rat testis; Cellular Location: Cytoplasm, Nucleus.

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Specifications

• Antigen: USP25
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Usp25
• Gene Alias: Deubiquitinating enzyme 25; mUSP25; Ubiquitin carboxyl-terminal hydrolase 25; ubiquitin specific peptidase 25; ubiquitin specific protease 25; ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; ubiquitin-specific-processing protease 25; USP on chromosome 21; USP21; Usp25
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29761, 304150, 30940
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.32 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P57080, Q9UHP3
• Gene Symbols: Usp25
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_0375283).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG