Invitrogen™ UPF1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA590296

Product Code. 16507771

Description

Positive Samples: 293T, SH-SY5Y, K-562, HL-60, Mouse liver; Cellular Location: Cytoplasm, Nucleus, P-body Immunogen sequence: QEVEIASVDA FQGREKDFII LSCVRANEHQ GIGFLNDPRR LNVALTRARY GVIIVGNPKA LSKQPLWNHL LNYYKEQKVL VEGPLNNLRE SLMQFSKPRK LVNTINPGAR FMTTAMYDAR EAIIPGSVYD RSSQGRPSSM YFQTHDQIGM ISAGPSHVAA MNIPIPFNLV MPPMPPPGYF GQANGPAAGR GTPKGKTGRG GRQKNRFGLP GPSQTNLPNS QASQDVASQP FSQGALTQGY ISMSQPSQMS QPGLSQPELS QDSYLGDEFK SQIDVALSQD STYQGERAYQ HGGVTGLSQY.

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm and was later shown to be a component of cytoplasmic P-bodies. Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery. Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively.

Specifications

• Antigen: UPF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UPF1
• Gene Alias: ATP-dependent helicase RENT1; B430202H16Rik; delta helicase; FLJ43809; FLJ46894; HUPF1; KIAA0221; LOW QUALITY PROTEIN: regulator of nonsense transcripts 1; mUpf1; Nonsense mRNA reducing factor 1; NORF1; pNORF1; PNORF-1; Regulator of nonsense transcripts 1; Regulator of nonsense transcripts 1 (Nonsense mRNA reducing factor 1) (NORF1) (Up-frameshift suppressor 1 homolog); Rent1; smg-2; smg-2 homolog, nonsense mediated mRNA decay factor; Upf1; UPF1 regulator of nonsense transcripts homolog; UPF1 regulator of nonsense transcripts homolog (yeast); UPF1 RNA helicase and ATPase; UPF1, RNA helicase and ATPase; Upflp; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; wu:fi40f07; wu:fj48a01; yeast Upf1p homolog; zgc:55472
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19704, 5976, 684558
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.85 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Gene Accession No.: Q92900, Q9EPU0
• Gene Symbols: UPF1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 819-1118 of human UPF1/RENT1 (NP_0029022).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG