R&D Systems™ Universal Chemiluminescent PARP Assay - Histone Coated Wells

Product Code. 30001325

Brand: R&D Systems™ 4676096K

Description

Chemiluminescent assay kit for candidate inhibitor screening and determination of IC₅₀ values of PARP inhibitors.

HT Universal 96-well PARP Assay Kits measure the incorporation of biotinylated poly(ADP-ribose) onto histone proteins in a strip well format. This assay is ideal for the screening of PARP inhibitors and determining IC₅₀ values. Important features of the assay include: 1) chemiluminescent, non-radioactive format; 2) higher throughput 96 test size; and 3) sensitivity down to 0.01 Units of PARP per well.

Kit Contents:

• PARP-HSA, 10 U/μL 50 μL
• 20X PARP Buffer (2 vials) 2 x 500 μL
• 10X PARP Cocktail (300 μlL)
• Histone-Coated White Strip Wells (96 Wells)
• 200 mM 3-Aminobenzamide (60 μL)
• 10X Strep-Diluent (2 mL)
• Strep-HRP (30 μL)
• PeroxyGlow™ A (6 mL)
• PeroxyGlow™ B (6 mL)
• 10X Activated DNA (300 μL)

Specifications

• Quantity: 96 Tests
• Content And Storage: Store the unopened product at -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.