Invitrogen™ UNG Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA585182

Product Code. 16362125

Description

Keep as concentrated solution. Predicted reactivity: Mouse (92%), Rat (94%), Xenopus laevis (83%), Chicken (86%), Chimpanzee (100%), Bovine (95%). Positive Control: Target recombinant protein. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB. The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis. Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type. Additionally, the expression of UNG is cell cycle regulated. The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells. UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.

Specifications

• Antigen: UNG
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ung
• Gene Alias: DGU; DKFZp781L1143; HIGM4; HIGM5; UDG; UNG; Ung1; UNG15; UNG2; unga; uracil DNA glycosylase; uracil DNA glycosylase a; uracil-DNA glycosylase; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; uracil-DNA glycosylase isoform UNG1; uracil-DNA glycosylase isoform UNG2; uracil-DNA glycosylase nuclear isoform; zgc:56102
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 393949, 7374
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Free Floating), Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P13051
• Gene Symbols: Ung, unga
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UNG. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Zebrafish
• Product Type: Antibody
• Isotype: IgG