Invitrogen™ UBXN1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA531809

Product Code. 13466327

Description

Recommended positive controls: 293T, A431, HeLa, NIH-3T3, JC, BCL-1. Predicted reactivity: Mouse (91%), Rat (87%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling (PubMed:23545497). Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1 (PubMed:25681446). Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B (PubMed:28152074). Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer (PubMed:20351172). Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. [UniProt].

Specifications

• Antigen: UBXN1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBXN1
• Gene Alias: 2B28; 4930455J02Rik; D19Ertd721e; mY33K; Protein 2B28; RGD1309471; SAKS1; SAPK substrate protein 1; T25529; UBA/UBX 33.3 kDa protein; UBX domain protein 1; UBX domain-containing protein 1; UBXD10; UBXN1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 225896, 51035
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.78 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q04323, Q922Y1
• Gene Symbols: UBXN1
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 106 and 297 of Human UBXN1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG