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Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), Biotin, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-6078-80
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin. Applications Reported: This eBioP4D1 (P4D1) antibody has been reported for use in immunoblotting (WB). Applications Tested: This eBioP4D1 (P4D1) antibody has been tested by immunoblotting of treated cell lines. This can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.
Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.Specifications
Ubiquitin | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P62979, P62982, P62983 | |
RPS27A | |
Affinity chromatography | |
RUO | |
100912032, 6233, 78294 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Western Blot | |
eBioP4D1 (P4D1) | |
Biotin | |
RPS27A | |
60S ribosomal protein L40, HUBCEP52, RPL40, Ubiquitin 52 amino acid fusion protein, Ubiquitin A52 residue ribosomal protein fusion product 1, Ubiquiti | |
Mouse | |
25 μg | |
Primary | |
All species | |
Antibody | |
IgG1 |
For Research Use Only.