Ub-K63 Mouse anti-Human, Clone: HWA4C4, eBioscience™

Mouse Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-6077-80


UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg

Disclaimers: For Research Use Only.

Product Code. 15287697

Quantity Price
1 £ 82.0 / 25µg
Estimated Shipment date
from Supplier 24-02-2017
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Description and Specification


Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias K63-linked polyubiquitin, Lysine63-linked poly ubiquitin chain
Host Species Mouse
Isotype IgG2a
Primary or Secondary Primary
Clone HWA4C4
Conjugate Unlabeled
Antigen Ub-K63
Applications Western Blotting
Quantity 25μg
Regulatory Status RUO
Species Reactivity Human
Storage Requirements Store at 2-8°C.
Monoclonal or Polyclonal Monoclonal
Concentration 0.5mg/mL

Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.

HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.