PeproTech TWEAK Polyclonal Antibody, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

Product Code	Brand	Quantity
17884013	PeproTech 500-P137G-50UG	50 μg
17874733	PeproTech 500-P137G-100UG	100 μg
17833903	PeproTech 500-P137G-1MG	1 mg

Description

AA Sequence of recombinant protein: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human TWEAK. Anti-Human TWEAK-specific antibody was purified by affinity chromatography employing an immobilized Human TWEAK matrix. Sandwich ELISA: To detect Human TWEAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TWEAK (500-P137GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TWEAK. Western Blot: To detect Human TWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TWEAK is 1.5-3.0 ng/lane, under either reducing or or non-reducing conditions.

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Specifications

• Antigen: TWEAK
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Goat
• Target Species: Human
• Gene Accession No.: O43508
• Gene ID (Entrez): 8742
• Immunogen: E.coli-derived Recombinant Human TWEAK.
• Purification Method: Antigen affinity chromatography
• Gene: Tnfsf12

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: APO3 ligand; APO3/DR3 ligand; APO3L; Dr3l; Dr3lg; LOW QUALITY PROTEIN: tumor necrosis factor ligand superfamily member 12; MGC129581; MGC20669; TNF superfamily member 12; TNF-related weak inducer of apoptosis; TNFSF12; TNLG4A; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; zgc:153941
• Gene Symbols: Tnfsf12
• Primary or Secondary: Primary
• Content And Storage: -20°C