Thermo Scientific™ TurboFect™ Transfection Reagent

Product Code.	Quantity	unitSize
12395613	200 μL	5 reactions
12331863	5 x 200 μL	5 reactions
15325016	1 mL	1mL
15391506	5 x 1 mL	5mL

Product Code. 12395613 Supplier Thermo Scientific™ Supplier No. R0533

Description

Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.

The Thermo Scientific™ TurboFect in vitro Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.

TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery into eukaryotic cells. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells. Transfection can be performed in the presence or absence of serum. TurboFect Reagent demonstrates superior transfection efficiency and minimal toxicity when compared to other lipid-based or polymer-based transfection reagents.

Highlights:

• High transfection efficiency of a wide variety of cell types
• No optimization required for most cell lines
• Excellent transfection efficiency in the presence or absence of serum
• Minimal cytotoxicity
• Ready-to-use – no need to reconstitute, dilute or manipulate

Recommended for:

Stable and transient transfection or co-transfection of DNA or RNA; Experiments with adherent or suspension cells, differentiated or undifferentiated cells; Gene expression studies and assays involving GFP or luciferase reporters

Specifications

• Type: Transfection Reagent
• Sufficient For: 100 Transfections
• Quantity: 200 μL
• Content And Storage: Upon receipt store at 4°C.
• Transfection Technique: Lipid-Based Transfection
• Product Line: TurboFect

Frequently Asked Questions (FAQs)

What is the stability of TurboFECT after being frozen?

TurboFect is a sterile solution of a proprietary cationic polymer in water. Its performance is not affected by a few freeze-thaw cycles. If TurboFECT arrives frozen or is accidentally stored at -20 degrees C, simply thaw and mix it thoroughly before use. For long-term storage, keep TurboFECT at 4 degrees C as recommended.

What are the advantages of using TurboFect Transfection Reagent (Cat. No. R0533) over other transfection reagents with the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186)?

The Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186) luciferase vectors were optimized with TurboFect. However, it should be feasible to replace TurboFect with another transfection reagent if your cells tolerate the transfection reagent better and give you acceptable transfection rates.

For Research Use Only. Not for use in diagnostic procedures.