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Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit
The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
Brand: Invitrogen™ A53815
Additional Details : Weight : 0.80000kg
Description
- A rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments
- Kit provides everything needed to achieve functional gene knockout and enrich the edited cells
- The donor produced will insert 3X STOP codons, a selection marker, and a fluorescent tag
- Simply provide two locus-specific primers to add homology arms and PCR-amplify the donor
- Easily design and order all necessary components to generate a knockout cell line
- Save up to 3 months with enrichment compared to single cell isolation and characterization
- Leverage a selection marker to obtain up to 100% edited cells
Each kit is designed to enable users to:
- Insert 3X STOP codons to knock out gene function
- Tag each allele with a different fluorophore (GFP or RFP) to verify bi-allelic knockout
- Utilize puromycin and blasticidin selection markers for bi-allelic knockout confirmation
- Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit:
- Four linear donor templates: GFP/puromycin resistance, GFP/blasticidin resistance, RFP/puromycin resistance, RFP/blasticidin resistance
- Phusion Flash High-Fidelity PCR Master Mix
- PCR cleanup columns and buffers
- Positive control primers for knockout of human HPRT1
Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper insertion of the knockout enrichment DNA donor. The Invitrogen TrueDesign Genome Editor web-based software does the design work for you:
- Identifies target gene transcript
- Selects a pre-designed or custom guide RNA
- Generates primers and enables easy ordering of all necessary components
Required reagents available via TrueDesign software (not included in kit):
- CRISPR guide RNA or TALENs designed for high-efficiency cleavage
- Oligos to PCR-amplify your donor and add appropriate homology arms
- Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
- Verification primers to validate proper insertion of the TrueTag DNA Donor
Also required and available for purchase separately or as part of TrueDesign workflow:
- TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
- CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent
Specifications
Gene Functional Analysis | |
10 reactions |
For Research Use Only. Not for use in diagnostic procedures