Invitrogen™ TRIzol™ Plus RNA Purification Kit and Phasemaker™ Tubes Complete System

This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes.

Brand:  Invitrogen™ A33254

Product Code. 15860612

Includes: One bottle of TRIzol Reagent 100 mL, 50 PureLink RNA Mini Columns and one bag of 100 Phasemaker Tubes

Description

This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes. The TRIzol Reagent offers superior lysis capability, even with difficult sample types. Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA.

The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers. It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer. Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%. It is an inert polymer that does not interfere with downstream experiments such as PCR. It works with fresh or frozen samples (tissue or blood). It also works with tissue samples treated previously with RNAlater. Invitrogen Phasemaker Tubes come already pre-dispensed. The tube size is 2 mL.

• Complete System includes TRIzol and Phasemaker Tubes
• Phasemaker Tubes are Pre-dispensed in 2 mL tubes
• Creates a tight seal between the aqueous and the organic phases
• Easier to pipette off the whole aqueous phase
• Increases yield up to 30%
• RNA integrity is not compromised
• The seal stays for longer periods of time, allowing to increase the number of samples per batch
• Inert, does not interfere with downstream experiments

Services

One year after receiving

Specifications

• Content And Storage:

  • 100 mL TRIzol Reagent; room temperature
  • 100 Phasemaker Tubes; room temperature
  • 1 PureLink RNA Mini Kit
  • 125 mL Lysis Buffer; room temperature
  • 50 mL Wash Buffer I; room temperature
  • 15 mL Wash Buffer II; room temperature
  • 15.5 mL RNase-free Water; room temperature
  • 50 PureLink Spin Cartridges with Collection Tubes; room temperature
  • 50 Collection Tubes; room temperature
  • 50 Recovery Tubes; room temperature
  
  

• Purification Time: 1 hr.
• Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: ≤350 μg

• Isolation Technology: Silica Spin Column, Organic Extraction
• Elution Volume: 30 to 200 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁷
  Cells: ≤10⁷
  Plant: ≤10⁷, ≤100 mg
  Tissue: ≤100 mg
  Yeast: ≤10⁷

For Research Use Only. Not for use in diagnostic procedures.