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Thermo Scientific Chemicals TRIS-glycine-SDS running buffer (10X), pH 8.5
Product Code. 15413679
Brand: Thermo Scientific Chemicals J61006.K7
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Description
Tris-Glycine SDS Running Buffer (10X) is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). It is ideally suited for protein gel analysis in biochemical, molecular biological, cell biological,and immunological research studies.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• TRIS-glycine-SDS running buffer (10X), is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins
Application
• This solution is the most commonly used buffer for protein gel analysis in several research studies
• It is also used to block membranes with 5% non-fat dry milk for western blotting
• TRIS-glycine-SDS running buffer is commonly used as both anode buffer and cathode buffer
• The use of SDS in the buffer helps keep the proteins linear. Glycine is an amino acid whose charge state plays an important role in the stacking gel
Specifications
Specifications
| Chemical Name or Material | TRIS-glycine-SDS running buffer (10X) |
| Physical Form | Liquid |
| Quantity | 4 L |
| Packaging | Plastic bottle |
| Solubility Information | It is soluble in water. |
Frequently Asked Questions (FAQs)
The TRIS-glycine-SDS running buffer (10X), pH 8.3 contains 0.25 M Tris base, 1.92 M glycine, and 1% SDS.
RUO – Research Use Only
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