Invitrogen™ H3K27me3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA585596

Product Code. 16302535

Description

Keep as concentrated solution. Predicted reactivity: Mouse (100%). Positive Control: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

Specifications

• Antigen: H3K27me3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: H3C1
• Gene Alias: H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3B.1; H3 histone, family 3C; H3 K27; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3.3A; H3.3b; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3b; h3-5; H3-53; H3-614; H3a; H3b; H3-B; H3C1; H3C10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3c8; H3f; H3-F; H3F1K; H3F2; H3F3; h3f3a; H3f3b; h3f3b.1; H3F3C; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3k27; H3Lys27me3; h3r; Hist1; Hist1h3a; HIST1H3B; Hist1h3c; Hist1h3d; HIST1H3E; HIST1H3F; hist1h3g; hist1h3g.L; HIST1H3H; Hist1h3i; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H3a; histone cluster 1, H3b; histone cluster 1, H3f; histone cluster 1, H3g protein L homeolog; histone cluster 1, H3h; histone cluster 2, H3a; histone cluster 2, H3c; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; histone H3.1; Histone H3.2; histone H3.3; Histone H3.3C; histone H3.5; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; histone H3/l; Histone H3/m; histone H3/o; Histone H3K27me3; histone variant H3.5; hypothetical protein LOC550262; M32461; methyl Histone 3; methyl Histone H3; PP781; Trimethyl H3; Tri-methyl H3; Trimethyl Histone H3; Tri-methyl Histone H3; Tri-Methyl-H3 K27; Tri-Methyl-H3 Lys27; Tri-Methyl-Histone H3 K27; Tri-Methyl-Histone H3 Lys27; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; XELAEV_18028537mg; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 126961, 260423, 319150, 319152, 333932, 360198, 8350, 8356, 8357, 8358, 8968, 97114
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P68431, P68433, P84228, Q71DI3
• Gene Symbols: H3C1, H3C10, H3C12, H3C14, H3C15, H3C2, H3C7
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG