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TRA-1-81 (Podocalyxin), APC, clone: TRA-1-81, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-8883-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15578206

  • £93.09 / 25 tests


Estimated Shipment date
from Supplier 26-06-2018
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Description

Description

Description: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TRA-1-81 antibody has been pre-titrated and tested by flow cytometric analysis of 2102Ep human embryonal carcinoma cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TRA-1-81 (podocalyxin)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Keratan sulfate antigen TRA1-81, TRA180
IgM
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Human
Flow Cytometry
TRA-1-81
APC
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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