TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15288107	Invitrogen 14-8883-80	25 μg
15298107	Invitrogen™ 14-8883-82	100 μg

Description

Description: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence. Applications Tested: This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonic carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

Specifications

• Clone: TRA-1-81
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Isotype: IgM
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Primary or Secondary: Primary
• Content And Storage: 4° C

For Research Use Only.