Invitrogen™ tPA Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA551927

Product Code. 15749880

Description

Immunogen sequence: KYSSEFCSTP ACSEGNSDCY FGNGSAYRGT HSLTESGASC LPWNSMILIG KVYTAQNPSA QALGLGKHNY CRNPDGDAKP WCHVLKNRRL TWEYCDVPSC STCGLRQYSQ PQFRIKGGLF Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 77%.

TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physio pathological events. It specifically cleaves the Arg- -Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.

Specifications

• Antigen: tPA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PLAT
• Gene Alias: 3.4.21.68; Alteplase; AU020998; AW212668; D8Ertd2e; PATISS; plasminogen activator, tissue; plasminogen activator, tissue type; plasminogen/activator kringle; PLAT; Reteplase; serine protease; t plasminogen activator; tissue plasminogen activator; tissue-type plasminogen activator; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B; TPA; t-PA; t-plasminogen activator
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5327
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P00750
• Gene Symbols: PLAT
• Immunogen: Recombinant protein corresponding to Human tPA. Recombinant protein control fragment (Product #RP-101047).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG