Invitrogen™ ToxBLAzer™ DualScreen Kit

Product Code. 11504936

Brand: Invitrogen™ K1139

Description

ToxBLAzer™ DualScreen Kit

ToxBLAzer™ DualScreen Kit provide the key advantage of combining the ratiometric reporter gene readout of beta-lactamase with a cytotoxicity readout in the same assay. Thus, ToxBLAzer™ DualScreen yields reporter readouts that normalize cytotoxic cellular responses, which is a necessity in screening applications which down regulate transcription. The ToxBLAzer™ substrate contains both the LiveBLAzer™-FRET B/G substrate and a proprietary cytotoxicity indicator. Both compounds readily enter the cell, where endogenous esterases rapidly convert them into fluorescent compounds Esterase activity and an intact cell membrane are required for detection of the indicator. The cytotoxicity indicator's fluorescence is red-shifted to ensure compatibility with the blue and green emissions of the LiveBLAzer™-FRET B/G substrate, allowing detection of both reporter gene activity and cytotoxicity in the same assay sample.

Advantages of ToxBLAzer™ DualScreen Kits:

Multiplexed readout results in rapid identification of false positives due to toxicity or lack of viable cells, thereby improving hit-picking efficiency

Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise

Single reagent addition eliminates need for follow up cytotoxicity assays, thereby increasing throughput while reducing sample and test compound consumption

Specifications

• Formulation: Liquid
• For Use With (Application): Protein biology, cell analysis, cellular imaging, cell-based reporter assays, cell-based and beta-lactamase assays, cell viability, proliferation and function, cell viability and cytotoxicity, drug discovery and development, target and lead identification and validation, kinase biology, kinase pathway analysis, G-protein coupled receptor biology, cell-based GPCR reporter assays, nuclear receptor biology, cell-based nuclear receptor reporter assays, pathway biology, cellular pathway analysis assays, other cellular pathway assay products, enzyme reporter assays, protein assays and analysis, beta-lactamase activity assays
• Includes: ToxBLAzer™ substrate, DMSO (for solution A), solution B, and solution C. ToxBLAzer™ substrate, DMSO (for solution A), solution B, and solution C should
• pH Range: 6 to 8
• Product Type: ToxBLAzer™ DualScreen Kit
• Quantity: 5mg

For Research Use Only. Not for use in diagnostic procedures.