Invitrogen™ TOX Monoclonal Antibody (TXRX10), eBioscience™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
17198525	Invitrogen™ 14-6502-37	2 mg
15237717	Invitrogen™ 14-6502-80	25 μg
15247717	Invitrogen™ 14-6502-82	100 μg
17108535	Invitrogen™ 14-6502-95	10 mg

Description

Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein. This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells. TOX expression in thymocytes is upregulated during beta-selection and positive selection. Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4^+ T cell development as a regulator of the CD4^loCD8^lo to CD4^+CD8^lo transition. Moreover, TOX has been shown to be involved in the development of NK and LTi cells. While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection. Applications Reported: This TXRX10 antibody has been reported for use in immunoprecipitation and immunoblotting (WB). Applications Tested: This TXRX10 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse thymocytes at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.

Specifications

• Antigen: TOX
• Concentration: 0.5 mg/mL
• Applications: Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O94900, Q66JW3
• Gene ID (Entrez): 252838, 9760
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: TXRX10
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human, Mouse
• Gene: TOX
• Gene Alias: 1700007F02Rik; KIAA0808; thymocyte selection associated high mobility group box; thymocyte selection-associated high mobility group box; thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; thymus high mobility group box protein TOX; TOX; TOX1
• Gene Symbols: TOX
• Product Type: Antibody