Invitrogen™ TNFSF4 (OX40L) Mouse ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15405353	Invitrogen™ EMTNFSF4	96 Tests
17105799	Invitrogen™ EMTNFSF4X5	5 x 96 Tests

Description

The Mouse TNFSF4 (OX40L) ELISA quantitates Ms TNFSF4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TNFSF4. Principle of the method The Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

Specifications

• Accession Number: P43488
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 30 pg/mL
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Specificity: This ELISA pair antibody detects mouse TNFSF4/OX40 Ligand and on cross reactivity with rmBAFF, rmEDA, rmGITR Ligand, rmFas Ligand, rmLIGHT, rmTWEAK, rhAPRIL, or rhTNFSF4.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 22164
• Intraassay CV: <10%
• Label or Dye: HRP
• Research Discipline: Inflammation
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.