Invitrogen™ TNFR2 Monoclonal Antibody (22221.311)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA124723

Product Code. 11534772

Description

Recommended positive controls: human peripheral blood lymphocytes. In Neutralization studies, at 0.5-1.0 μg/mL, this antibody will block 50% of the bioactivity due to 0.3 μg/mL recombinant human sTNF RII, in the presence of 0.25 ng/mL TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells. Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

Specifications

• Antigen: TNFR2
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and no preservative
• Gene Accession No.: P20333
• Gene Symbols: Tnfrsf1b
• Immunogen: Recombinant human tumor necrosis factor receptor II, expressed in Escherichia coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Neutralization, Immunocytochemistry
• Clone: 22221.311
• Conjugate: Unconjugated
• Gene: Tnfrsf1b
• Gene Alias: CD120b; Etanercept; p75; p75 TNF receptor; p75 TNFR; p75TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TBP-2; TBPII; TNF receptor beta chain; TNF receptor superfamily member 1B; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNFR II; Tnfr-1; TNFR1B; TNFR2; TNF-R2; Tnfr-2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1b, membrane form; tumor necrosis factor receptor superfamily, member 1B; tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 7133
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid