PeproTech TNFR1 (soluble) Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17824243	PeproTech 500-P143-50UG	50 μg
17854783	PeproTech 500-P143-100UG	100 μg
17844043	PeproTech 500-P143-1MG	1 mg

Description

AA Sequence of recombinant protein: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type I matrix. Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type I (500-P143Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I. Western Blot: To detect Human sTNF Receptor Type I by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

Specifications

• Antigen: TNFR1 (soluble)
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P19438
• Gene ID (Entrez): 7132
• Immunogen: E.coli-derived Recombinant Human sTNF Receptor Type I.
• Purification Method: Antigen affinity chromatography
• Gene: Tnfrsf1a

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: CD120a; CD120a antigen; FPF; hypothetical protein; metalloproteinase inhibitor 4; MGC19588; MS5; n-smase activation assoc. factor; p55; p55 TNF receptor; p55-R; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TBP1; TBPI; TIMP metallopeptidase inhibitor 4; Timp4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4; TNF receptor alpha chain; TNF receptor superfamily member 1A; TNF-alphaR1; TNF-alpha-R1; TNFalpha-R1; TNFAR; TNFR; TNF-R; TNFR I; TNFR1; TNF-R1; Tnfr-1; TNFR1-d2; Tnfr-2; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRp55; Tnfrsf1a; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor superfamily member 1A, membrane form; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; Tumor necrosis factor-binding protein 1
• Gene Symbols: Tnfrsf1a
• Primary or Secondary: Primary
• Content And Storage: -20°C