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Invitrogen™ TNFR1 (Soluble) Monkey ELISA Kit
Description
Includes
- Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sTNF-R I
- HRP-Conjugate anti-monkey sTNF-R I monoclonal antibody
- Monkey sTNF-R I Standard concentrate, 12 U/ml upon dilution
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Stop Solution (1M Phosphoric acid)
- Blue-Dye
- Green-Dye
- Adhesive Films
The Monkey Tumor Necrosis Factor Receptor 1 (Mk TNFR1) ELISA quantitates Mk TNFR1 in monkey(baboon, cynomolgus) serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk TNFR1. Principle of the method The Monkey TNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Specifications
Specifications
| Assay Sensitivity | 0.06 U/mL |
| Conjugate | HRP |
| Sample Type | Baboon Serum, Cell Culture Supernatant, Cynomolgus Serum |
| Target Species | Monkey |
| For Use With (Application) | ELISA |
| Quantity | 10 x 96 Tests |
| Synonym | CD120a, TNFR60, TNF Receptor superfamily member 1A |
| Target | sCD120a (sTNF Receptor I) |
For Research Use Only
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