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TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-7349-81
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody. Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MAb11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.Specifications
TNF alpha | |
Monoclonal | |
0.2mg/mL; 5μL (0.25μg)/test | |
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer | |
Mouse | |
RUO | |
Human | |
IgG1 κ |
Flow Cytometry | |
MAb11 | |
PE | |
Tumor Necrosis Factor alpha | |
50 μg | |
Primary | |
Store at 2-8°C. Do not freeze. Light-sensitive material. |
For Research Use Only.