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TNF alpha, clone: TN3-19.12, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7423-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278077

  • £49.88 / 50µg


Estimated Shipment
26-07-2018
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Description

Description

Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, ELISPOT, IVCCA, and ELISA. Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry. Functional Grade purified TN3-19.12, cat. 16-7423, is recommended for use in functional assays. For ELISPOT capture, the alternative clone, 1F3F3D4 (cat # 16-7325), is recommended.Applications Tested: The TN3-19.12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the biotinylated anti-mouse/rat TNF alpha polyclonal antibody (13-7341) for detection and recombinant mouse TNF alpha (14-8321) or rat TNF alpha as the standard. Product 14-8320 is a bioactive protein, but is not recommended for rat TNF alpha ELISA.] A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF alpha) or 5000 - 30 pg/mL (rat TNF alpha) should be included in each ELISA plate. Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.The Functional Grade purified TN3-19.12 antibody has been tested for neutralization of TNF alpha bioactivity. The fluorochrome-conjugated TN3-19.12 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis and can be used at 20 μL per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TNF alpha
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P06804, P16599
DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
MurineþRat
Cytokine NeutralizationþELISAþFlow Cytometry
TN3-19.12
Unconjugated
Tnf
Purified
Tnf
IgG
50μg
Store at 2-8°C.
Primary
21926
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