TIGIT Mouse anti-Mouse, Brilliant Violet 605, Clone: 1G9, BD Optibuild™

Mouse Monoclonal Antibody

Brand:  BD Biosciences 744212

Product Code. 15898849

Description

The 1G9 monoclonal antibody specifically binds to T cell immunoreceptor with Ig and ITIM domains (TIGIT) which is also known as, V-set and transmembrane domain-containing protein 3 (Vstm3). TIGIT is a 26 kDa, type I transmembrane glycoprotein that belongs to the poliovirus receptor (PVR) family. TIGIT is expressed on activated and memory CD4+ T cells and CD8+ T cells, follicular T helper cells, regulatory T cells, and NK cells. Its ligands include the poliovirus receptor (PVR, aka, CD155), poliovirus receptor-related protein 2 (PVRL2, aka, CD112), and poliovirus receptor-related protein 3 (PVRL3, CD113). TIGIT functions as a negative regulator of T cell and NK cell responses including proliferation, cytotoxicity, and the production of proinflammatory cytokines. TIGIT-deficient mice are reportedly more susceptible to developing autoimmune diseases in certain model systems.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.

Specifications

• Antigen: TIGIT
• Clone: 1G9
• Conjugate: Brilliant Violet 605
• Gene Alias: Tigit, Vstm3, T cell immunoreceptor with Ig and ITIM domains
• Immunogen: Mouse TIGIT Recombinant Protein
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1 κ