Invitrogen™ TID1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA527226

Product Code. 13421377

Description

Recommended positive controls: HepG2, HCT116. Predicted reactivity: Mouse (87%), Rat (87%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

Specifications

• Antigen: TID1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNAJA3
• Gene Alias: 1200003J13Rik; 1810053A11Rik; C81173; DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ heat shock protein family (Hsp40) member A3; dnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Dnaja3; HCA57; Hepatocellular carcinoma-associated antigen 57; hTID-1; mTid-1; Tid1; Tid-1; Tid1l; Tumorous imaginal discs protein Tid56 homolog
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9093
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.46 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q96EY1
• Gene Symbols: DNAJA3
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TID1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG