Invitrogen™ TID1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA596554

Product Code. 16559391

Description

Immunogen sequence: AKGVNKEFTV NIMDTCERCN GKGNEPGTKV QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIIISPCV VCRGAGQAKQ KKRVMIPVPA GVEDGQTVRM PVGKREIFIT FRVQKSPVFR RDGADIHSDL FISIAQALLG GTARAQGLYE TINVTIPPGT QTDQKIRMGG KGIPRINSYG YGDHYIHIKI RVPKRLTSRQ QSLILSYAED ETDVEGTVNG VTLTSSGGST MDSSAGSKAR REAGEDEEGF LSKLKKMFTS; Positive Samples: BT-474, Jurkat, 293T, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver; Cellular Location: Cell junction, Cytoplasm, Mitochondrion matrix, Peripheral membrane protein, cytosol, postsynaptic cell membrane, synapse.

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

Specifications

• Antigen: TID1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNAJA3
• Gene Alias: 1200003J13Rik; 1810053A11Rik; C81173; DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ heat shock protein family (Hsp40) member A3; dnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Dnaja3; HCA57; Hepatocellular carcinoma-associated antigen 57; hTID-1; mTid-1; Tid1; Tid-1; Tid1l; Tumorous imaginal discs protein Tid56 homolog
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 360481, 83945, 9093
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.4 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q96EY1, Q99M87
• Gene Symbols: DNAJA3
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_0051383).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG