Thymidylate Synthase Antibody (TMS715), Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18133105	Novus Biologicals NBP2-34446-0.02MG	0.02 mg
18127532	Novus Biologicals NBP2-34446-0.1MG	0.1 mg

Description

Thymidylate Synthase Monoclonal specifically detects Thymidylate Synthase in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: Thymidylate Synthase
• Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P04818
• Gene ID (Entrez): 7298
• Immunogen: Recombinant human thymidylate synthase
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 36 kDa

• Clone: TMS715
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Cycle and Replication, DNA Repair, DNA replication Transcription Translation and Splicing
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: EC 2.1.1.45, HsT422, MGC88736, thymidylate synthase, thymidylate synthetase, TMSTsase, TSase, TSHST422
• Gene Symbols: TYMS
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.