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PeproTech Thrombopoietin Polyclonal Antibody, PeproTech®, Invitrogen™
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Product Code. 17815283
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50 μg
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50µg
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17815283 50 μg 50µg
17825283 100 μg 100µg
17864963 1 mg 1mg
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Product Code. 17815283 Supplier PeproTech Supplier No. 500P49G50UG

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Description

Goat Polyclonal Antibody

AA Sequence of recombinant protein: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human TPO. Anti-Human TPO-specific antibody was purified by affinity chromatography employing an immobilized Human TPO matrix. Sandwich ELISA: To detect Human TPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TPO (500-P49GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TPO. Western Blot: To detect Human TPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Thrombopoietin
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene THPO
Gene Accession No. P40225
Gene Alias C-mpl ligand; Megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; Mgdf; MKCSF; ML; MPL ligand; Mpllg; MSA; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; TDH2A; THCYT1; THPO; Thrombopoietin; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; Tpo; TPX
Gene Symbols THPO
Host Species Goat
Immunogen E.coli-derived Recombinant Human TPO.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7066
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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