Invitrogen™ Thioredoxin 1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA534742

Product Code. 15388611

Description

Recommended positive controls: H1299, HeLaS3. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

Specifications

• Antigen: Thioredoxin 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TXN
• Gene Alias: ADF; ATL-derived factor; AW550880; DKFZp686B1993; MGC61975; RP11-427L11.1; SASP; surface-associated sulphydryl protein; testicular tissue protein Li 199; thioredoxin; thioredoxin 1; thioredoxin delta 3; TRDX; TRX; TRX1; Txn; TXN delta 3; Txn1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22166, 7295
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.69 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P10599, P10639
• Gene Symbols: TXN, Txn1
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Thioredoxin. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG