Thermo Scientific™ Thermo-Start™ Taq DNA Polymerase PCR Kts and Master Mxes

Hot-start modified for PCR applications requiring maximum specificity and sensitivity to detect rare or complex targets and allow for room-temperature reaction setup.

Brand:  Thermo Scientific™ AB-1057/B

Product Code. 11887662

Description

Thermo Scientific™ Thermo-Start mixes are hot-start modified for PCR applications requiring maximum specificity and sensitivity, to detect rare or complex targets; also allows for room-temperature reaction setup.

Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands.

• Hot-Start modification ensures stringent specificity
• Safe for room temperature reaction setup
• Enhanced enzyme stability for increased yield and sensitivity
• Advanced buffering system minimizes PCR optimization steps
• High Performance Buffer formulation for GC-rich and other complex templates

Thermo-Start is available in a range of formats and pack sizes to suit any workflow.

Format Options:

• Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffer, and 25mM MgCl₂ solution, each in separate vials. dNTPs must be purchased separately

• Complete PCR Kits include all the components needed to perform PCR, including an HPLC-purified dNTP mix. Complete PCR kits are a convenient option that still allows for MgCl₂ optimization

• Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format as they significantly reduce assay set-up steps and minimize the risk of sample contamination

• Premixed MgCl₂ Kits have MgCl₂ premixed into the PCR buffer and will be at 1.5mM concentration in the final reaction

• High Performance Buffer Kits are specially formulated for GC-rich or other complex targets with a high degree of secondary structure

Compatible with:

Thermo-Start Taq Reddymix Kits and Master Mixes

Recommended for:

Amplifying fragments up to 5kb; Hot-Start PCR; RT-PCR; Sequencing; Multiplexing; Genotyping; Library Construction; Cloning; Pathogen Detection; Single Cell PCR

Specifications

• Content And Storage: Store at -20°C for long-term storage. Stable at 4°C for up to four weeks. Avoid freeze-thaw cycles.
• Quantity: 4000 x 25µL Rxns
• Compatibility: Thermo-Start Taq Reddymix Kits and Master Mixes

• Description: Thermo-Start DNA Polymerase Enzyme Kit w/High Performance Buffer (for GC-rich)
• For Use With (Application): Cloning
• Concentration: 5U/µL

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.