Invitrogen™ Human IGF-I Recombinant Protein

Product Code.	Quantity	unitSize
11584940	1 mg	1000µg

Product Code. 11584940 Supplier Invitrogen™ Supplier No. RP10931

Description

Recombinant Protein

This is a recombinant protein suitable for use as a control in various applications. Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles. Reconstitute in sterile water not less than 100 μg/mL.

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

Specifications

• Accession Number: P05019
• For Use With (Application): Bioactivity
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3479
• Molecular Weight (g/mol): 7.6 kDa
• Name: Human IGF-I
• Purification Method: Chromatography
• Quantity: 1 mg
• Source: E. coli
• Immunogen: N/A
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: C730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-C
• Common Name: IGF1
• Gene Symbol: IGF1
• Biological Activity: ED50 < 1.0 ng/mL; determined by the dose-dependent proliferation of mouse Balb/c 3T3 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Human IGF-1 recombinant protein contains 70 amino acids
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: ED50 < 1.0 ng/mL; determined by the dose-dependent proliferation of mouse Balb/c 3T3 cells.
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >97% by SDS-PAGE and analyzed by silver stain.