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Invitrogen™ Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ RP8628

Code : LE

Additional Details : Weight : 0.01000kg

Product Code. 11589141

  • £296.00 / 50µg
Estimated Shipment: 26-04-2024
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Description

Description

Fibroblast Growth Factor basic (human) Recombinant Protein, RP-8628, from Invitrogen.

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization. Recombinant human FGF-b 154 (FGF-2) is a non-glycosylated protein, containing 154 amino acids, with a molecular weight of 17.2 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1ng/mL. The specific activity of this protein is 1x10^6 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Specifications

Specifications

P09038
Control
2247
Human FGF basic
50μg
-20°C, Avoid Freeze/Thaw Cycles
H-FGF-b-154
FGF2
Unconjugated
E. coli
Lyophilized
purified
0.1mg/mL
10mM sodium phosphate with 75mM NaCl and no preservative; pH 7.5
∽17.2
7.5
E. coli
RUO
Human FGF basic
Human
Hu
Recombinant
Recombinant
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