Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Product Code. 15264756

Brand: Thermo Scientific™ F170S

Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

• Direct PCR reduces time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
• Specially engineered Phire DNA polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Notes

• The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

• Concentration: 2X
• Content And Storage: • 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL
  • Dilution Buffer, 5 mL
  • DNARelease Additive, 3 x 100 μL
  • 25 μM Control Primer Mix, 40 μL
  • Nuclease-free water, 2 x 1.25 mL
  • 50 μg O'GeneRuler Express DNA Ladder
  
  Store at -20°C.
• GC-Rich PCR Performance: Medium
• PCR Method: Direct PCR
• Polymerase: Phire Hot Start II DNA Polymerase
• Reaction Speed: Fast
• Includes Label or Dye: Yes
• Product Type: Direct PCR Master Mix
• Quantity: 250 reactions
• Shipping Condition: Wet or Dry Ice
• For Use With (Application): Genotyping, Hot-start PCR
• Hot Start: Built-In Hot Start
• No. of Reactions: 250 Reactions
• Overhang: Blunt
• Reaction Format: SuperMix or Master Mix
• Sample Type: Tissue
• Starting Material: DNA

Safety and Handling

Product Identifier

• Phire Tissue Direct PCR Master Mix/DNARelease Additive

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1

Hazard Statement

• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Proteinase, tritirachium album serine

For Research Use Only. Not for use in diagnostic procedures.