Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Product Code.	Sufficient For	Unit Size
13595260	5000 Reactions	5000 reactions
13585260	500 Reactions	500 reactions
13575260	250 Reactions	250 reactions
13505270	250 Reactions	1250 reactions

Artikelnummer. 13595260 Lieferant Thermo Scientific™ Lieferanten-Nr. K0994

Beschreibung

Includes

Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

Spezifikation

• Concentration: 2X
• Content And Storage: Contains:
  • 4 x 12.5 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions
  • 2 x 30 mL Nuclease-Free Water
  
  Store at -20°C.
• Detection Method: SYBR
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
• Product Line: Luminaris
• Product Type: qPCR Master Mix
• Quantity: 5,000 reactions
• Sufficient For: 5000 Reactions
• For Use With (Application): Gene Expression
• Fidelity (vs. Taq): 2 X
• No. of Reactions: 5000 Reactions
• Sample Type: DNA, dsDNA

For Research Use Only. Not for use in diagnostic procedures.