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Thermo Scientific™ EpiJET Bisulfite Conversion Kit
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Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR.
The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step. This step can be performed following long or short protocol, depending on the downstream application of modified DNA. The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps. The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing.
Highlights
- High conversion efficiency and specificity (≥99%)
- Flexibility, provided by short DNA conversion protocol
- Pure DNA, suitable for downstream applications and long-term storage
Applications
Converted DNA can be used in:
- PCR
- qPCR
- Combined Bisulfite Restriction Analysis (COBRA)
- Sequencing
Includes
- Modification Reagent
- Modification Solution I
- Modification Solution II
- Binding Buffer for Bisulfite Conversion Kit
- Wash buffer (conc.) for Bisulfite Conversion Kit
- Desulfonation Buffer (conc.)
- Elution Buffer
- DNA Purification Micro Columns and Collection Tubes
Specifications
Specifications
| Quantity | 50 reactions |
| Product Type | Bisulfite Conversion Kit |
| Product Line | EpiJET™ |
Safety and Handling
- EpiJET Bisulfite Conversion Kit
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Reproductive toxicity Category 1
- Skin corrosion/irritation Category 1 A
- Skin corrosion/irritation Category 1 B
- Skin corrosion/irritation Category 2
- H226-Flammable liquid and vapour.
- H312-Harmful in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H360D-May damage the unborn child.
- P201-Obtain special instructions before use.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P310-Immediately call a POISON CENTER/doctor/.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Dimethylformamide, Sodium metabisulfite,Sodium hydroxide
For Research Use Only. Not for use in diagnostic procedures.
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