Invitrogen™ Themis Monoclonal Antibody (1TMYS), PE, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen™ 12-5918-80

Product Code. 15507056

Description

Description: This 1TMYS monoclonal antibody reacts with mouse Thymocyte-expressed molecule involved in selection (Themis), also known as Grb2-associating protein (Gasp). Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing. The 1TMYS antibody recognizes isoforms 1, 2, 3, and 4, but does not recognize isoform 5. Applications Reported: This 1TMYS antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 1TMYS antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes or splenocytes using the intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cell...

Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.

Specifications

• Antigen: Themis
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8BGW0
• Gene Symbols: Themis
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 210757
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 1TMYS
• Conjugate: PE
• Gene: Themis
• Gene Alias: bA325O24.3; bA325O24.4; C6orf190; C6orf207; E430004N04Rik; FLJ40584; Gasp; GRB2-associated protein; Grb2-associating protein; MGC163388; Protein THEMIS; Protein thylex; RGD1560849; signaling phosphoprotein specific for T cells; SPOT; THEMIS; thylex; thymocyte expressed molecule involved in selection; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; Tsepa
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ