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PeproTech TGF alpha Polyclonal Antibody, PeproTech®, Invitrogen™

Product Code. 17844293
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100 μg
50 μg
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100µg
1mg
50µg
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17844293 50 μg 50µg
17874373 100 μg 100µg
17804643 1 mg 1mg
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Product Code. 17844293 Supplier PeproTech Supplier No. 500P1650UG

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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TGF-alpha matrix. Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TGF-alpha (500-P16Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
TRUSTED_SUSTAINABILITY

Specifications

Antigen TGF alpha
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene TGFA
Gene Accession No. P01135
Gene Alias EGF-like TGF; ETGF; I79_019067; LOC100009150; Protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF α; TGFA; TGFAA; TGFalpha; TGF-alpha; TGFα; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1
Gene Symbols TGFA
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human TGF-alpha.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7039
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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