TFF2 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen 500-P312BT-1MG

Product Code. 17874813

Description

AA Sequence of recombinant protein: AEKPSPCQCS RLSPHNRTNC GFPGITSDQC FDNGCCFDSS VTGVPWCFHP LPKQESDQCV MEVSDRRNCG YPGISPEECA SRKCCFSNFI FEVPWCFFPK SVEDCHY. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TFF-2. Anti-Human TFF-2-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human TFF-2 by sandwich ELISA (using 100 μL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human TFF-2 (500-P312) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TFF-2. Western Blot: To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human TFF-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P312BT-1MG will be provided as 2 x 500 μg

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion. SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.

Specifications

• Antigen: TFF2
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Tff2
• Gene Alias: mSP; pancreatic spasmolytic polypeptide; PSP; SML1; SP; spasmolysin; Spasmolytic polypeptide; spasmolytic protein 1; Tff2; Trefoil factor 2; trefoil factor 2 (spasmolytic protein 1)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7032
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q03403
• Gene Symbols: Tff2
• Immunogen: E.coli-derived, 12.0 kDa Recombinant Human TFF-2
• Quantity: 2 x 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized